› 2qtv

X-ray diffraction
2.5Å resolution

Structure of Sec23-Sar1 complexed with the active fragment of Sec31

Released:
DOI: 10.2210/pdb2qtv/pdb
PDB entry 2qtv coloured by chain, front view.
PDB entry 2qtv coloured by chain, side view.
PDB entry 2qtv coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31.
Bi X, Mancias JD, Goldberg J
Dev Cell 13 635-645 (2007)
PMID: 17981133

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-147301 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein transport protein SEC23 Chain: A
Molecule details ›
Chain: A
Length: 772 amino acids
Theoretical weight: 85.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15303 (Residues: 2-768; Coverage: 100%)
Gene names: P9705.14, SEC23, YPR181C
Sequence domains:
Structure domains:
Small COPII coat GTPase SAR1 Chain: B
Molecule details ›
Chain: B
Length: 167 amino acids
Theoretical weight: 18.79 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P20606 (Residues: 23-189; Coverage: 88%)
Gene names: SAR1, YPL218W
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Protein transport protein SEC31 Chain: D
Molecule details ›
Chain: D
Length: 49 amino acids
Theoretical weight: 5.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P38968 (Residues: 899-947; Coverage: 4%)
Gene names: D1229, SEC31, WEB1, YDL195W
Sequence domains: Protein transport protein SEC31

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 94.748Å b: 133.213Å c: 82.041Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.262
Expression system: Spodoptera frugiperda

Quick links

Citations

45 review citations

Organization of the ER-Golgi interface for membrane traffic control.
Brandizzi et al. (2013)
44 more

13 mentions without citation

Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31.
Bi et al. (2007)
12 more