2b0q Summary


PDB entry 2b0q (supersedes 1l8u)

Crystal Structure Of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Neomycin B Complex

The structure was published by Fong, D.H. and Berghuis, A.M., in 2002 in a paper entitled "Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.7 Å and deposited in 2005.

The experimental data on which the structure is based was not deposited.

The PDB entry contains the structure of Aminoglycoside 3'-phosphotransferase. This molecule has the UniProt identifier P0A3Y5 (KKA3_ENTFL)search. The sample contained 263 residues which is 100% of the natural sequence. Out of 263 residues 263 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Aminoglycoside 3'-phosphotransferase P0A3Y5 (2-264) (KKA3_ENTFL)search Enterococcus faecalissearch 100% 263 100%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P0A3Y5 (2 - 264) Aminoglycoside 3'-phosphotransferase Enterococcus faecalis

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P0A3Y5) APH phosphotransferasessearch Phosphorylase Kinase; domain 1search, Aminoglycoside 3'-phosphotransferase; Chain: A, domain 2search PF01636: Phosphotransferase enzyme familysearch

Chain ID Biological process (GO) Molecular function (GO)
A (P0A3Y5) response to antibioticsearch phosphorylationsearch ATP bindingsearch kanamycin kinase activitysearch kinase activitysearch transferase activitysearch nucleotide bindingsearch transferase activity, transferring phosphorus-containing groupssearch phosphotransferase activity, alcohol group as acceptorsearch

Chain InterPro annotation
A Aminoglycoside phosphotransferasesearch Protein kinase-like domainsearch Aminoglycoside 3-phosphotransferasesearch