› 1ud0

X-ray diffraction
3.45Å resolution

CRYSTAL STRUCTURE OF THE C-TERMINAL 10-kDA SUBDOMAIN OF HSC70

Released:
DOI: 10.2210/pdb1ud0/pdb
PDB entry 1ud0 coloured by chain, front view.
PDB entry 1ud0 coloured by chain, side view.
PDB entry 1ud0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of the C-terminal 10-kDa subdomain of Hsc70.
Chou CC, Forouhar F, Yeh YH, Shr HL, Wang C, Hsiao CD
J Biol Chem 278 30311-6 (2003)
PMID: 12773536

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-158924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock cognate 71 kDa protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 113 amino acids
Theoretical weight: 12.45 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63018 (Residues: 542-646; Coverage: 16%)
Gene names: Hsc70, Hsc73, Hspa8
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P6122
Unit cell:
a: 117.479Å b: 117.479Å c: 163.777Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.249 0.309
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The structural and functional diversity of Hsp70 proteins from Plasmodium falciparum.
Shonhai et al. (2007)
1 more

1 mention without citation

Hsp70 oligomerization is mediated by an interaction between the interdomain linker and the substrate-binding domain.
Aprile et al. (2013)