› 1oij

X-ray diffraction
2.1Å resolution

Crystal structure of the alkylsulfatase AtsK, a non-heme Fe(II) alphaketoglutarate dependent Dioxygenase in complex with alphaketoglutarate

Released:
DOI: 10.2210/pdb1oij/pdb
PDB entry 1oij coloured by chain, front view.
PDB entry 1oij coloured by chain, side view.
PDB entry 1oij coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of the alkylsulfatase AtsK: insights into the catalytic mechanism of the Fe(II) alpha-ketoglutarate-dependent dioxygenase superfamily.
Müller I, Kahnert A, Pape T, Sheldrick GM, Meyer-Klaucke W, Dierks T, Kertesz M, Usón I
Biochemistry 43 3075-88 (2004)
PMID: 15023059

Function and Biology Details

Reaction catalysed: 
A primary alkyl sulfate ester + 2-oxoglutarate + O(2) = an aldehyde + succinate + CO(2) + sulfate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194415 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Alpha-ketoglutarate-dependent sulfate ester dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.28 KDa
Source organism: Pseudomonas putida
UniProt:
  • Canonical: Q9WWU5 (Residues: 1-301; Coverage: 100%)
Gene name: atsK
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like
Alpha-ketoglutarate-dependent sulfate ester dioxygenase Chains: B, D
Molecule details ›
Chains: B, D
Length: 301 amino acids
Theoretical weight: 33.25 KDa
Source organism: Pseudomonas putida
UniProt:
  • Canonical: Q9WWU5 (Residues: 1-301; Coverage: 100%)
Gene name: atsK
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like
Alpha-ketoglutarate-dependent sulfate ester dioxygenase Chain: C
Molecule details ›
Chain: C
Length: 301 amino acids
Theoretical weight: 33.28 KDa
Source organism: Pseudomonas putida
UniProt:
  • Canonical: Q9WWU5 (Residues: 1-301; Coverage: 100%)
Gene name: atsK
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand AKG 4 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 72.447Å b: 144.876Å c: 160.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.215

Quick links

Citations

12 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
11 more

1 mention without citation

Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases.
Müller et al. (2006)