› 1v5c

X-ray diffraction
2Å resolution

The crystal structure of the inactive form chitosanase from Bacillus sp. K17 at pH3.7

Released:
DOI: 10.2210/pdb1v5c/pdb
PDB entry 1v5c coloured by chain, front view.
PDB entry 1v5c coloured by chain, side view.
PDB entry 1v5c coloured by chain, top view.
Source organism: Bacillus sp. (in: Bacteria)
Primary publication:
Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17.
Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, Yatsunami R, Nakamura S, Takénaka A
J Mol Biol 343 785-95 (2004)
PMID: 15465062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 43.61 KDa
Source organism: Bacillus sp. (in: Bacteria)
UniProt:
  • Canonical: Q9ALZ1 (Residues: 49-434; Coverage: 91%)
Gene name: csn45
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I222
Unit cell:
a: 85.063Å b: 91.145Å c: 131.524Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.208

Quick links

Citations

10 review citations

Production of chitooligosaccharides and their potential applications in medicine.
Aam et al. (2010)
9 more

6 mentions without citation

Non-homologous isofunctional enzymes: a systematic analysis of alternative solutions in enzyme evolution.
Omelchenko et al. (2010)
5 more