Conserved Site

Glycoside hydrolase, family 8, conserved site (IPR019834)

Short name: Glyco_hydro_8_CS

Description

O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PMID: 7624375, PMID: 8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycoside hydrolase family 8 GH8 comprises enzymes with several known activities; endoglucanase (EC:3.2.1.4); lichenase (EC:3.2.1.73); chitosanase (EC:3.2.1.132). These enzymes were formerly known as cellulase family D [PMID: 2806912].

The most conserved region in these enzymes is a stretch of about 20 residues that contains two conserved aspartates. The first asparatate is thought [PMID: 8805535] to act as the nucleophile in the catalytic mechanism. This region as a signature pattern in this entry.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns