Searching for ZAP70 using EBI-search

In this scenario we will help Melissa find out more about ZAP70 by using EBI search.

To use EBI Search, simply go to the EMBL-EBI homepage and type ZAP70 in the search box. Once the search has finished scroll down to see the results and data snippets.

The main panel of the results pane is divided into three sections:

• Filters on the left cluster databases into groups which can also be used to refine your results.
• The top section, Gene & protein summaries, has summary pages for frequently studied species. These summaries draw from numerous data resources at EMBL-EBI to bring together commonly sought information such as gene and protein sequences, expression patterns and three-dimensional structural information.
• Scroll down further and you can browse the full list of results.

Next we will have a look at the ‘Gene & protein summaries’ for ZAP70. Click on the link to go through to this page.

On the left hand side you will see several tabs that guide you to summary information about the gene, its expression, the protein, its structure and any supporting literature.

Explore these tabs to find the answers to the following questions:

• Which chromosome is human ZAP70 on?
• In which of the following tissues is baseline expression data for ZAP70 NOT available? Blood, Brain, Gut Epithelium, Endometrium.
• How many isoforms of ZAP70 have been identified?
• Is ZAP70 is a protein tyrosine kinase that spans the plasma membrane?
• Are there any rodents with 1:1 orthologues of human ZAP70?

Check your answers in the quiz on the next page.