PDBsum entry 8jd2

Membrane protein

PDB id: 8jd2

Contents

Protein chains

750 a.a.

749 a.a.

Ligands

GLU ×2

NAG ×2

PDB id:

8jd2

Name:

Membrane protein

Title:

Cryo-em structure of g protein-free mglu2-mglu3 heterodimer in acc state

Structure:

Metabotropic glutamate receptor 2,peptidyl-prolyl cis-trans isomerase fkbp1a. Chain: 2. Synonym: mglur2,ppiase fkbp1a,12 kda fk506-binding protein,12 kda fkbp,fkbp-12,calstabin-1,fk506-binding protein 1a,fkbp-1a, immunophilin fkbp12,rotamase. Engineered: yes. Metabotropic glutamate receptor 3,serine/threonine-protein kinase mtor.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: grm2, gprc1b, mglur2, fkbp1a, fkbp1, fkbp12. Expressed in: mammal environmental sample. Expression_system_taxid: 1092587. Gene: grm3, gprc1c, mglur3, mtor. Expression_system_taxid: 1092587

Authors:

X.Wang,M.Wang,T.Xu,Y.Feng,S.Han,S.Lin,Q.Zhao,B.Wu

Key ref:

X.Wang et al. Structural insights into dimerization and activation mglu2-Mglu3 and mglu2-Mglu4 heterodimers.. Cell res, . PubMed id: 37286794

Date:

12-May-23 Release date: 21-Jun-23

Protein chain

P62942  (FKB1A_HUMAN) -  Peptidyl-prolyl cis-trans isomerase FKBP1A from Homo sapiens

Seq: 108 a.a.

Struc: 750 a.a.*

Protein chain

Q14416  (GRM2_HUMAN) -  Metabotropic glutamate receptor 2 from Homo sapiens

Seq: 872 a.a.

Struc: 750 a.a.

Protein chain

A0A8V8TRG9  (A0A8V8TRG9_HUMAN) -  Serine/threonine-protein kinase mTOR from Homo sapiens

Seq: 2478 a.a.

Struc: 749 a.a.*

Protein chain

Q14832  (GRM3_HUMAN) -  Metabotropic glutamate receptor 3 from Homo sapiens

Seq: 879 a.a.

Struc: 749 a.a.

* PDB and UniProt seqs differ at 793 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain 2: E.C.5.2.1.8 - peptidylprolyl isomerase.
• Reaction: [protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)
• Enzyme class 2: Chain 3: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = NAG) matches with 41.38% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = NAG) matches with 41.38% similarity) + ADP + H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site