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PDBsum entry 6j4z
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Transcription/RNA/DNA
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PDB id
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6j4z
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Contents |
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1414 a.a.
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1157 a.a.
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263 a.a.
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168 a.a.
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213 a.a.
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84 a.a.
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171 a.a.
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133 a.a.
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111 a.a.
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66 a.a.
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113 a.a.
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45 a.a.
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102 a.a.
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275 a.a.
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97 a.a.
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80 a.a.
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103 a.a.
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95 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural insight into nucleosome transcription by RNA polymerase ii with elongation factors.
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Authors
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H.Ehara,
T.Kujirai,
Y.Fujino,
M.Shirouzu,
H.Kurumizaka,
S.I.Sekine.
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Ref.
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Science, 2019,
363,
744-747.
[DOI no: ]
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PubMed id
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Abstract
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RNA polymerase II (RNAPII) transcribes chromosomal DNA that contains multiple
nucleosomes. The nucleosome forms transcriptional barriers, and nucleosomal
transcription requires several additional factors in vivo. We demonstrate that
the transcription elongation factors Elf1 and Spt4/5 cooperatively lower the
barriers and increase the RNAPII processivity in the nucleosome. The
cryo-electron microscopy structures of the nucleosome-transcribing RNAPII
elongation complexes (ECs) reveal that Elf1 and Spt4/5 reshape the EC downstream
edge and intervene between RNAPII and the nucleosome. They facilitate RNAPII
progression through superhelical location SHL(-1) by adjusting the nucleosome in
favor of the forward progression. They suppress pausing at SHL(-5) by preventing
the stable RNAPII-nucleosome interaction. Thus, the EC overcomes the nucleosomal
barriers while providing a platform for various chromatin functions.
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