PDBsum entry 4b9b

Transferase

PDB id: 4b9b

Contents

Protein chains

(+ 2 more) 436 a.a.

Ligands

PLP ×8

GOL ×10

Metals

_CL ×8

_CA ×4

Waters ×4574

PDB id:

4b9b

Name:

Transferase

Title:

The structure of the omega aminotransferase from pseudomonas aeruginosa

Structure:

Beta-alanine-pyruvate transaminase. Chain: a, b, c, d, e, f, g, h. Synonym: aminotransferase. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.64Å R-factor: 0.177 R-free: 0.219

Authors:

C.Sayer,M.N.Isupov,A.Westlake,J.A.Littlechild

Key ref:

C.Sayer et al. (2013). Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity. Acta Crystallogr D Biol Crystallogr, 69, 564-576. PubMed id: 23519665 DOI: 10.1107/S0907444912051670

Date:

03-Sep-12 Release date: 27-Mar-13

Protein chains

Q9I700  (BAUA_PSEAE) -  Beta-alanine--pyruvate aminotransferase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 448 a.a.

Struc: 436 a.a.

Enzyme reactions

• Enzyme class: E.C.2.6.1.18 - beta-alanine--pyruvate transaminase.
• Reaction: 3-oxopropanoate + L-alanine = beta-alanine + pyruvate

3-oxopropanoate (Bound ligand (Het Group name = GOL) matches with 71.43% similarity) + L-alanine = beta-alanine + pyruvate

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1107/S0907444912051670

Acta Crystallogr D Biol Crystallogr 69:564-576 (2013)

PubMed id: 23519665

Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity.

C.Sayer, M.N.Isupov, A.Westlake, J.A.Littlechild.

ABSTRACT

No abstract given.