PDBsum entry 3sfd

Oxidoreductase

PDB id: 3sfd

Contents

Protein chains

613 a.a.

239 a.a.

138 a.a.

102 a.a.

Ligands

FAD

OAA

FES

SF4

F3S

HEM

PCI

Waters ×130

PDB id:

3sfd

Name:

Oxidoreductase

Title:

Crystal structure of porcine mitochondrial respiratory complex ii bound with oxaloacetate and pentachlorophenol

Structure:

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial. Chain: a. Synonym: flavoprotein subunit of complex ii, fp. Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial. Chain: b. Synonym: iron-sulf protein, iron-sulfur subunit of complex ii, ip. Succinate dehydrogenase cytochrome b560 subunit,

Source:

Sus scrofa. Pigs. Organism_taxid: 9823. Organism_taxid: 9823

Resolution:

2.61Å R-factor: 0.244 R-free: 0.286

Authors:

Q.J.Zhou,Y.J.Zhai,M.Liu,F.Sun

Key ref:

Q.Zhou et al. (2011). Thiabendazole inhibits ubiquinone reduction activity of mitochondrial respiratory complex II via a water molecule mediated binding feature. Protein Cell, 2, 531-542. PubMed id: 21822798 DOI: 10.1007/s13238-011-1079-1

Date:

13-Jun-11 Release date: 07-Sep-11

Protein chain

Q0QF01  (SDHA_PIG) -  Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial from Sus scrofa

Seq: 664 a.a.

Struc: 613 a.a.

Protein chain

Q007T0  (SDHB_PIG) -  Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial from Sus scrofa

Seq: 280 a.a.

Struc: 239 a.a.

Protein chain

D0VWV4  (C560_PIG) -  Succinate dehydrogenase cytochrome b560 subunit, mitochondrial from Sus scrofa

Seq: 169 a.a.

Struc: 138 a.a.

Protein chain

A5GZW8  (DHSD_PIG) -  Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial from Sus scrofa

Seq: 159 a.a.

Struc: 102 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.1.1.5.- - ?????
• Enzyme class 3: Chains A, B: E.C.1.3.5.1 - succinate dehydrogenase.

Pathway:

• Reaction: a quinone + succinate = fumarate + a quinol

quinone (Bound ligand (Het Group name = PCI) matches with 53.85% similarity) + succinate (Bound ligand (Het Group name = OAA) matches with 88.89% similarity) = fumarate + quinol

• Cofactor: FAD; Iron-sulfur

FAD (Bound ligand (Het Group name = FAD) corresponds exactly) Iron-sulfur

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1007/s13238-011-1079-1

Protein Cell 2:531-542 (2011)

PubMed id: 21822798

Thiabendazole inhibits ubiquinone reduction activity of mitochondrial respiratory complex II via a water molecule mediated binding feature.

Q.Zhou, Y.Zhai, J.Lou, M.Liu, X.Pang, F.Sun.

ABSTRACT

No abstract given.