PDBsum entry 8e7c

Viral protein, hydrolase/inhibitor

PDB id

8e7c

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Contents

			Protein chains

					301 a.a.

			Ligands

					V2M ×2


					GOL ×13


					DMS ×7

			Waters ×243

PDB id:

8e7c

Links

Name:

Viral protein, hydrolase/inhibitor

Title:

Crystal structure of porcine deltacoronavirus (hku-15) mpro with pfizer intravenous inhibitor pf-00835231

Structure:

Main protease. Chain: a, b. Engineered: yes

Source:

Porcine deltacoronavirus. Organism_taxid: 1586324. Gene: orf1ab. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.45Å

R-factor:

0.201

R-free:

0.235

Authors:

A.M.Shaqra,C.A.Schiffer

Key ref:

S.N.Zvornicanin et al. (2023). Crystal structures of inhibitor-Bound main protease f delta- And gamma-Coronaviruses. Viruses, 15, . PubMed id: 36992489

Date:

23-Aug-22

Release date:

29-Mar-23

PROCHECK

	Headers

	References

Protein chains

A0A0E3N825 (A0A0E3N825_9NIDO) - ORF1ab polyprotein from Porcine deltacoronavirus

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					6267 a.a. 301 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class 2:

E.C.2.1.1.57 - methyltransferase cap1.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)- (2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H⁺

5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA	+	S-adenosyl-L-methionine	=	5'-end (N(7)-methyl 5'-triphosphoguanosine)- (2'-O-methyl-ribonucleoside) in mRNA	+	S-adenosyl-L-homocysteine	+	H(+)

Enzyme class 3:

E.C.2.7.7.50 - mRNA guanylyltransferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a 5'-end diphospho-ribonucleoside in mRNA + GTP + H⁺ = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate

5'-end diphospho-ribonucleoside in mRNA	+	GTP	+	H(+)	=	5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA	+	diphosphate

Enzyme class 4:

E.C.3.4.19.12 - ubiquitinyl hydrolase 1.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme class 5:

E.C.3.6.4.12 - Dna helicase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

ATP + H2O = ADP + phosphate + H⁺

ATP	+	H2O	=	ADP	+	phosphate	+	H(+)

Enzyme class 6:

E.C.3.6.4.13 - Rna helicase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

ATP + H2O = ADP + phosphate + H⁺

ATP	+	H2O	=	ADP	+	phosphate	+	H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site