PDBsum entry 6ipc

Oxidoreductase

PDB id: 6ipc

Contents

Protein chains

(+ 2 more) 162 a.a.

(+ 2 more) 143 a.a.

Ligands

MPD ×2

Waters ×17

PDB id:

6ipc

Name:

Oxidoreductase

Title:

Non-native human ferritin 8-mer

Structure:

Ferritin heavy chain. Chain: a, b, c, e, f, g, h, i, j, k, m, n, o, p. Synonym: ferritin h subunit,cell proliferation-inducing gene 15 protein. Engineered: yes. Mutation: yes. Ferritin heavy chain. Chain: d, l. Synonym: ferritin h subunit,cell proliferation-inducing gene 15

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fth1, fth, fthl6, ok/sw-cl.84, pig15. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

4.44Å R-factor: 0.210 R-free: 0.277

Authors:

J.C.Zang,H.Chen,G.Zhao

Key ref:

J.Zang et al. (2019). Disulfide-mediated conversion of 8-mer bowl-like protein architecture into three different nanocages. Nat Commun, 10, 778. PubMed id: 30770832 DOI: 10.1038/s41467-019-08788-9

Date:

03-Nov-18 Release date: 13-Mar-19

Protein chains

P02794  (FRIH_HUMAN) -  Ferritin heavy chain from Homo sapiens

Seq: 183 a.a.

Struc: 162 a.a.*

Protein chains

P02794  (FRIH_HUMAN) -  Ferritin heavy chain from Homo sapiens

Seq: 183 a.a.

Struc: 143 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P: E.C.1.16.3.1 - ferroxidase.
• Reaction: 4 Fe²⁺ + O2 + 4 H⁺ = 4 Fe³⁺ + 2 H2O
• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1038/s41467-019-08788-9

Nat Commun 10:778 (2019)

PubMed id: 30770832

Disulfide-mediated conversion of 8-mer bowl-like protein architecture into three different nanocages.

J.Zang, H.Chen, X.Zhang, C.Zhang, J.Guo, M.Du, G.Zhao.

ABSTRACT

Constructing different protein nanostructures with high-order discrete architectures by using one single building block remains a challenge. Here, we present a simple, effective disulfide-mediated approach to prepare a set of protein nanocages with different geometries from single building block. By genetically deleting an inherent intra-subunit disulfide bond, we can render the conversion of an 8-mer bowl-like protein architecture (NF-8) into a 24-mer ferritin-like nanocage in solution, while selective insertion of an inter-subunit disulfide bond into NF-8 triggers its conversion into a 16-mer lenticular nanocage. Deletion of the same intra-subunit disulfide bond and insertion of the inter-subunit disulfide bond results in the conversion of NF-8 into a 48-mer protein nanocage in solution. Thus, in the laboratory, simple mutation of one protein building block can generate three different protein nanocages in a manner that is highly reminiscent of natural pentamer building block originating from viral capsids that self-assemble into protein assemblies with different symmetries.