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PDBsum entry 6ipc
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Oxidoreductase
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PDB id
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6ipc
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Contents |
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(+ 2 more)
162 a.a.
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(+ 2 more)
143 a.a.
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References listed in PDB file
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Key reference
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Title
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Disulfide-Mediated conversion of 8-Mer bowl-Like protein architecture into three different nanocages.
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Authors
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J.Zang,
H.Chen,
X.Zhang,
C.Zhang,
J.Guo,
M.Du,
G.Zhao.
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Ref.
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Nat Commun, 2019,
10,
778.
[DOI no: ]
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PubMed id
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Abstract
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Constructing different protein nanostructures with high-order discrete
architectures by using one single building block remains a challenge. Here, we
present a simple, effective disulfide-mediated approach to prepare a set of
protein nanocages with different geometries from single building block. By
genetically deleting an inherent intra-subunit disulfide bond, we can render the
conversion of an 8-mer bowl-like protein architecture (NF-8) into a 24-mer
ferritin-like nanocage in solution, while selective insertion of an
inter-subunit disulfide bond into NF-8 triggers its conversion into a 16-mer
lenticular nanocage. Deletion of the same intra-subunit disulfide bond and
insertion of the inter-subunit disulfide bond results in the conversion of NF-8
into a 48-mer protein nanocage in solution. Thus, in the laboratory, simple
mutation of one protein building block can generate three different protein
nanocages in a manner that is highly reminiscent of natural pentamer building
block originating from viral capsids that self-assemble into protein assemblies
with different symmetries.
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