PDBsum entry 5t1v

Hydrolase

PDB id: 5t1v

Contents

Protein chains

158 a.a.

163 a.a.

PDB id:

5t1v

Name:

Hydrolase

Title:

Crystal structure of zika virus ns2b-ns3 protease in apo-form.

Structure:

Ns2b-ns3 protease,ns2b-ns3 protease. Chain: a, b. Ec: 3.4.21.91,3.6.1.15,3.6.4.13,2.1.1.56,2.1.1.57,2.7.7.48,3.4.21.91, 3.6.1.15,3.6.4.13,2.1.1.56,2.1.1.57,2.7.7.48. Engineered: yes

Source:

Zika virus. Zikv. Organism_taxid: 64320. Strain: mr 766. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

3.10Å R-factor: 0.229 R-free: 0.288

Authors:

S.Nocadello,S.H.Light,G.Minasov,L.Shuvalova,A.A.Cardona-Correa, I.Ojeda,J.Vargas,M.E.Johnson,H.Lee,W.F.Anderson,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

S.Nocadello et al. Crystal structure of zika virus ns2b-Ns3 protease in apo-Form.. To be published, .

Date:

22-Aug-16 Release date: 07-Sep-16

Protein chain

Q32ZE1  (POLG_ZIKV) -  Genome polyprotein from Zika virus

Seq: 3419 a.a.

Struc: 158 a.a.*

Protein chain

Q32ZE1  (POLG_ZIKV) -  Genome polyprotein from Zika virus

Seq: 3419 a.a.

Struc: 163 a.a.*

* PDB and UniProt seqs differ at 33 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.1.1.56 - mRNA (guanine-N(7))-methyltransferase.
• Reaction: a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine
• Enzyme class 2: Chains A, B: E.C.2.1.1.57 - methyltransferase cap1.
• Reaction: a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)- (2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H⁺
• Enzyme class 3: Chains A, B: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 4: Chains A, B: E.C.3.4.21.91 - flavivirin.
• Reaction: Selective hydrolysis of Xaa-Xaa-|-Xbb bonds in which each of the Xaa can be either Arg or Lys and Xbb can be either Ser or Ala.
• Enzyme class 5: Chains A, B: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺
• Enzyme class 6: Chains A, B: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site