PDBsum entry 5lmn

Ribosome

PDB id: 5lmn

Contents

Protein chains

234 a.a.

206 a.a.

208 a.a.

150 a.a.

101 a.a.

155 a.a.

138 a.a.

127 a.a.

98 a.a.

120 a.a.

124 a.a.

117 a.a.

60 a.a.

88 a.a.

83 a.a.

99 a.a.

73 a.a.

80 a.a.

99 a.a.

24 a.a.

71 a.a.

168 a.a.

DNA/RNA

Metals

_MG ×127

_ZN ×2

PDB id:

5lmn

Name:

Ribosome

Title:

Structure of bacterial 30s-if1-if3-mRNA translation pre-initiation complex (state-1a)

Structure:

16s ribosomal RNA. Chain: a. 30s ribosomal protein s2. Chain: b. 30s ribosomal protein s3. Chain: c. 30s ribosomal protein s4. Chain: d. 30s ribosomal protein s5.

Source:

Thermus thermophilus hb8. Organism_taxid: 300852. Thermus thermophilus (strain hb8 / atcc 27634 / dsm 579). Strain: hb8 / atcc 27634 / dsm 579. Gene: infa, ttha1669. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: infc, ttha0551.

Authors:

T.Hussain,J.L.Llacer,B.T.Wimberly,V.Ramakrishnan

Key ref:

T.Hussain et al. (2016). Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation. Cell, 167, 133. PubMed id: 27662086 DOI: 10.1016/j.cell.2016.08.074

Date:

01-Aug-16 Release date: 05-Oct-16

Protein chain

P80371  (RS2_THET8) -  Small ribosomal subunit protein uS2 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 256 a.a.

Struc: 234 a.a.

Protein chain

P80372  (RS3_THET8) -  Small ribosomal subunit protein uS3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 239 a.a.

Struc: 206 a.a.

Protein chain

P80373  (RS4_THET8) -  Small ribosomal subunit protein uS4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 209 a.a.

Struc: 208 a.a.

Protein chain

Q5SHQ5  (RS5_THET8) -  Small ribosomal subunit protein uS5 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 162 a.a.

Struc: 150 a.a.

Protein chain

Q5SLP8  (RS6_THET8) -  Small ribosomal subunit protein bS6 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 101 a.a.

Struc: 101 a.a.

Protein chain

P17291  (RS7_THET8) -  Small ribosomal subunit protein uS7 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 156 a.a.

Struc: 155 a.a.

Protein chain

P0DOY9  (RS8_THET8) -  Small ribosomal subunit protein uS8 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 138 a.a.

Struc: 138 a.a.

Protein chain

P80374  (RS9_THET8) -  Small ribosomal subunit protein uS9 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 128 a.a.

Struc: 127 a.a.

Protein chain

Q5SHN7  (RS10_THET8) -  Small ribosomal subunit protein uS10 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 105 a.a.

Struc: 98 a.a.

Protein chain

P80376  (RS11_THET8) -  Small ribosomal subunit protein uS11 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 129 a.a.

Struc: 120 a.a.

Protein chain

Q5SHN3  (RS12_THET8) -  Small ribosomal subunit protein uS12 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 132 a.a.

Struc: 124 a.a.

Protein chain

P80377  (RS13_THET8) -  Small ribosomal subunit protein uS13 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 126 a.a.

Struc: 117 a.a.

Protein chain

P0DOY6  (RS14Z_THET8) -  Small ribosomal subunit protein uS14 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 61 a.a.

Struc: 60 a.a.

Protein chain

Q5SJ76  (RS15_THET8) -  Small ribosomal subunit protein uS15 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 89 a.a.

Struc: 88 a.a.

Protein chain

Q5SJH3  (RS16_THET8) -  Small ribosomal subunit protein bS16 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 88 a.a.

Struc: 83 a.a.

Protein chain

P0DOY7  (RS17_THET8) -  Small ribosomal subunit protein uS17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 105 a.a.

Struc: 99 a.a.

Protein chain

Q5SLQ0  (RS18_THET8) -  Small ribosomal subunit protein bS18 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 88 a.a.

Struc: 73 a.a.

Protein chain

Q5SHP2  (RS19_THET8) -  Small ribosomal subunit protein uS19 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 93 a.a.

Struc: 80 a.a.

Protein chain

P80380  (RS20_THET8) -  Small ribosomal subunit protein bS20 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 106 a.a.

Struc: 99 a.a.

Protein chain

Q5SIH3  (RSHX_THET8) -  Small ribosomal subunit protein bTHX from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 27 a.a.

Struc: 24 a.a.

Protein chain

Q5SHR1  (IF1_THET8) -  Translation initiation factor IF-1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 72 a.a.

Struc: 71 a.a.

Protein chain

Q5SKU2  (IF3_THET8) -  Translation initiation factor IF-3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 171 a.a.

Struc: 168 a.a.

DNA/RNA chains

U-G-G-A-G-A-G-U-U-U-G-A-U-C-C-U-G-G-C-U-C-A-G-G-G-U-G-A-A-C-G-C-U-G-G-C-G-G-C- ... 1514 bases

A-G-G-C-A-A-G-G-A-G-G-U-A-A-A-A-A-U-G-U 20 bases

DOI no: 10.1016/j.cell.2016.08.074

Cell 167:133 (2016)

PubMed id: 27662086

Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.

T.Hussain, J.L.Llácer, B.T.Wimberly, J.S.Kieft, V.Ramakrishnan.

ABSTRACT

In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.