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PDBsum entry 5lmn
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Contents |
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234 a.a.
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206 a.a.
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208 a.a.
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150 a.a.
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101 a.a.
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155 a.a.
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138 a.a.
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127 a.a.
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98 a.a.
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120 a.a.
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124 a.a.
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117 a.a.
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60 a.a.
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88 a.a.
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83 a.a.
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99 a.a.
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73 a.a.
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80 a.a.
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99 a.a.
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24 a.a.
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71 a.a.
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168 a.a.
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References listed in PDB file
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Key reference
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Title
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Large-Scale movements of if3 and tRNA during bacterial translation initiation.
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Authors
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T.Hussain,
J.L.Llácer,
B.T.Wimberly,
J.S.Kieft,
V.Ramakrishnan.
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Ref.
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Cell, 2016,
167,
133.
[DOI no: ]
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PubMed id
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Abstract
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In bacterial translational initiation, three initiation factors (IFs 1-3) enable
the selection of initiator tRNA and the start codon in the P site of the 30S
ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy
(cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator
tRNA, mRNA, and IFs 1-3, representing different steps along the initiation
pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing
their activities. IF2 positions a domain in an extended conformation appropriate
for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo
large conformational changes to facilitate the accommodation of the
formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon
recognition.
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