PDBsum entry 4l5o

Transferase

PDB id: 4l5o

Contents

Protein chains

217 a.a.

Ligands

GSH ×3

SO4 ×6

Metals

_ZN ×2

Waters ×255

PDB id:

4l5o

Name:

Transferase

Title:

Crystal structure of 26 kda gst d26h mutant of clonorchis sinensis

Structure:

Putative glutathione transferase. Chain: a, b, c. Engineered: yes. Mutation: yes

Source:

Clonorchis sinensis. Oriental liver fluke. Organism_taxid: 79923. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.09Å R-factor: 0.204 R-free: 0.248

Authors:

Y.J.Chung,Y.H.Han

Key ref:

Y.H.Han et al. (2013). Crystal structures of 26kDa Clonorchis sinensis glutathione S-transferase reveal zinc binding and putative metal binding. Biochem Biophys Res Commun, 438, 457-461. PubMed id: 23916611 DOI: 10.1016/j.bbrc.2013.07.102

Date:

11-Jun-13 Release date: 11-Sep-13

Protein chains

Q25595  (Q25595_CLOSI) -  glutathione transferase from Clonorchis sinensis

Seq: 218 a.a.

Struc: 217 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.5.1.18 - glutathione transferase.
• Reaction: RX + glutathione = an S-substituted glutathione + a halide anion + H⁺

RX (Bound ligand (Het Group name = GSH) corresponds exactly) + glutathione = S-substituted glutathione + halide anion + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1016/j.bbrc.2013.07.102

Biochem Biophys Res Commun 438:457-461 (2013)

PubMed id: 23916611

Crystal structures of 26kDa Clonorchis sinensis glutathione S-transferase reveal zinc binding and putative metal binding.

Y.H.Han, S.J.Hong, H.K.Cheong, Y.J.Chung.

ABSTRACT

The crystal structures of CsGST in two different space groups revealed that Asp26 and His79 coordinate a zinc ion. In one space group, His46 of an adjacent molecule participates in the coordination within 2.0Å. In the other space group, Asp26, His79 and a water molecule coordinate a zinc ion. The CsGST-D26H structure showed that four histidine residues - His26 and His79 from one molecule and the same residues from a symmetry-related neighboring molecule - coordinate a zinc ion. The coordinated zinc ions are located between two molecules and mediate molecular contacts within the crystal.