PDBsum entry 3d9c

Hydrolase

PDB id: 3d9c

Contents

Protein chain

282 a.a. *

Ligands

ZYZ

Waters ×47

* Residue conservation analysis

PDB id:

3d9c

Name:

Hydrolase

Title:

Crystal structure ptp1b complex with aryl seleninic acid

Structure:

Tyrosine-protein phosphatase non-receptor type 1. Chain: a. Fragment: unp residues 1-283. Synonym: protein-tyrosine phosphatase 1b, ptp-1b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ptpn1, ptp1b. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.30Å R-factor: 0.218 R-free: 0.274

Authors:

M.Abdo,S.Liu,B.Zhou,C.D.Walls,S.Knapp,Z.-Y.Zhang

Key ref:

M.Abdo et al. (2008). Seleninate in place of phosphate: irreversible inhibition of protein tyrosine phosphatases. J Am Chem Soc, 130, 13196-13197. PubMed id: 18781746

Date:

27-May-08 Release date: 23-Sep-08

Protein chain

P18031  (PTN1_HUMAN) -  Tyrosine-protein phosphatase non-receptor type 1 from Homo sapiens

Seq: 435 a.a.

Struc: 282 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

J Am Chem Soc 130:13196-13197 (2008)

PubMed id: 18781746

Seleninate in place of phosphate: irreversible inhibition of protein tyrosine phosphatases.

M.Abdo, S.Liu, B.Zhou, C.D.Walls, L.Wu, S.Knapp, Z.Y.Zhang.

ABSTRACT

A homotyrosine based seleninic acid irreversibly inhibits protein tyrosine phosphatases by forming a covalent selenosulfide linkage with the active site cysteine sulfhydryl specifically. The details of the event are revealed by model synthetic studies and by kinetic, mass spectrometric, and crystallographic characterization.