PDBsum entry 3n6m

Transferase

PDB id

3n6m

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Contents

			Protein chain

					462 a.a.

			Ligands

					GTP

			Metals

					_NI

			Waters ×230

PDB id:

3n6m

Links

Name:

Transferase

Title:

Crystal structure of ev71 rdrp in complex with gtp

Structure:

RNA-dependent RNA polymerase. Chain: a. Engineered: yes

Source:

Human enterovirus 71. Organism_taxid: 39054. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å

R-factor:

0.197

R-free:

0.249

Authors:

Y.Wu,Z.Y.Lou,Y.Miao,Y.Yu,Z.H.Rao

Key ref:

Y.Wu et al. (2010). Structures of EV71 RNA-dependent RNA polymerase in complex with substrate and analogue provide a drug target against the hand-foot-and-mouth disease pandemic in China. Protein Cell, 1, 491-500. PubMed id: 21203964 DOI: 10.1007/s13238-010-0061-7

Date:

26-May-10

Release date:

15-Jun-11

PROCHECK

	Headers

	References

Protein chain

D3K0N8 (D3K0N8_HE71) - Genome polyprotein from Human enterovirus 71

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					2193 a.a. 462 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class 2:

E.C.2.7.7.48 - RNA-directed Rna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

RNA(n)	+	ribonucleoside 5'-triphosphate	=	RNA(n+1)	+	diphosphate

Enzyme class 3:

E.C.3.4.22.28 - picornain 3C.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Enzyme class 4:

E.C.3.4.22.29 - picornain 2A.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Enzyme class 5:

E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

ribonucleoside 5'-triphosphate	+	H2O	=	ribonucleoside 5'-diphosphate	+	phosphate	+	H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1007/s13238-010-0061-7	Protein Cell 1:491-500 (2010)
PubMed id: 21203964

Structures of EV71 RNA-dependent RNA polymerase in complex with substrate and analogue provide a drug target against the hand-foot-and-mouth disease pandemic in China.
Y.Wu, Z.Lou, Y.Miao, Y.Yu, H.Dong, W.Peng, M.Bartlam, X.Li, Z.Rao.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22388738

X.Wang, W.Peng, J.Ren, Z.Hu, J.Xu, Z.Lou, X.Li, W.Yin, X.Shen, C.Porta, T.S.Walter, G.Evans, D.Axford, R.Owen, D.J.Rowlands, J.Wang, D.I.Stuart, E.E.Fry, and Z.Rao (2012).
A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71.

Nat Struct Mol Biol, 19, 424-429.

PDB codes:

3vbf 3vbh 3vbo 3vbr 3vbs 3vbu

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.