PDBsum entry 3vbs

Virus

PDB id: 3vbs

Contents

Protein chains

297 a.a.

245 a.a.

242 a.a.

58 a.a.

Ligands

SPH

PDB id:

3vbs

Name:

Virus

Title:

Crystal structure of human enterovirus 71

Structure:

Genome polyprotein, capsid protein vp1. Chain: a. Synonym: ev71. Genome polyprotein, capsid protein vp2. Chain: b. Synonym: ev71. Genome polyprotein, capsid protein vp3. Chain: c. Synonym: ev71.

Source:

Human enterovirus 71. Organism_taxid: 39054. Strain: fuyang, anhui. P.R.C/17.08/1. Other_details: grown in vero cells. Other_details: grown in vero cells

Resolution:

3.00Å R-factor: 0.273 R-free: 0.285

Authors:

X.Wang,W.Peng,J.Ren,Z.Hu,J.Xu,Z.Lou,X.Li,W.Yin,X.Shen,C.Porta, T.S.Walter,G.Evans,D.Axford,R.Owen,D.J.Rowlands,J.Wang,D.I.Stuart, E.E.Fry,Z.Rao

Key ref:

X.Wang et al. (2012). A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71. Nat Struct Biol, 19, 424-429. PubMed id: 22388738

Date:

02-Jan-12 Release date: 29-Feb-12

Protein chain

B2ZUN0  (B2ZUN0_HE71) -  Genome polyprotein from Human enterovirus 71

Seq: 2193 a.a.

Struc: 297 a.a.*

Protein chain

B2ZUN0  (B2ZUN0_HE71) -  Genome polyprotein from Human enterovirus 71

Seq: 2193 a.a.

Struc: 245 a.a.

Protein chain

B2ZUN0  (B2ZUN0_HE71) -  Genome polyprotein from Human enterovirus 71

Seq: 2193 a.a.

Struc: 242 a.a.

Protein chain

B2ZUN0  (B2ZUN0_HE71) -  Genome polyprotein from Human enterovirus 71

Seq: 2193 a.a.

Struc: 58 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: Chains A, B, C, D: E.C.3.4.22.28 - picornain 3C.
• Reaction: Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 4: Chains A, B, C, D: E.C.3.4.22.29 - picornain 2A.
• Reaction: Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 5: Chains A, B, C, D: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Nat Struct Biol 19:424-429 (2012)

PubMed id: 22388738

A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71.

X.Wang, W.Peng, J.Ren, Z.Hu, J.Xu, Z.Lou, X.Li, W.Yin, X.Shen, C.Porta, T.S.Walter, G.Evans, D.Axford, R.Owen, D.J.Rowlands, J.Wang, D.I.Stuart, E.E.Fry, Z.Rao.

ABSTRACT

No abstract given.