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PDBsum entry 2vl5
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Immune system
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PDB id
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2vl5
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Contents |
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* Residue conservation analysis
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PDB id:
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Immune system
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Title:
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Structure of anti-collagen type ii fab ciic1
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Structure:
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Ciic1 anticollagen fab. Chain: a, c. Ciic1 anticollagen fab. Chain: b, d
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Source:
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Mus musculus. Mouse. Organism_taxid: 10090. Strain: dba/1. Cell_line: monoclonal b-cell hybridoma. Organ: spleen. Cell: monoclonal b-cell hybridoma. Cell: monoclonal b-cell hybridoma
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Resolution:
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2.10Å
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R-factor:
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0.216
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R-free:
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0.289
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Authors:
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H.Uysal,B.Sehnert,K.S.Nandakumar,U.Boiers,H.Burkhardt,R.Holmdahl, M.M.Thunnissen
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Key ref:
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H.Uysal
et al.
(2008).
The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: structure to function analysis.
Mol Immunol,
45,
2196-2204.
PubMed id:
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Date:
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08-Jan-08
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Release date:
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18-Mar-08
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PROCHECK
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Headers
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References
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Mol Immunol
45:2196-2204
(2008)
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PubMed id:
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The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: structure to function analysis.
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H.Uysal,
B.Sehnert,
K.S.Nandakumar,
U.Böiers,
H.Burkhardt,
R.Holmdahl,
M.M.Thunnissen.
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ABSTRACT
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Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic
autoantibody, which induces arthritis in mice. We crystallized and solved the
structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction
between the CDR regions of one Fab to the CH1 domain of another Fab, which
resembles an antibody-antigen interaction. ELISA experiments confirmed the
cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment
to other anti-collagen antibodies which are of different isotypes and epitope
specificity. The rheumatoid factor like reactivity of CIIC1 antibody together
with its collagen type II specificity may explain the pathogenicity of this
antibody.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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K.S.Nandakumar
(2010).
Pathogenic antibody recognition of cartilage.
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Cell Tissue Res,
339,
213-220.
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H.Uysal,
R.Bockermann,
K.S.Nandakumar,
B.Sehnert,
E.Bajtner,
A.Engström,
G.Serre,
H.Burkhardt,
M.M.Thunnissen,
and
R.Holmdahl
(2009).
Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis.
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J Exp Med,
206,
449-462.
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PDB codes:
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M.J.Rowley,
K.S.Nandakumar,
and
R.Holmdahl
(2008).
The role of collagen antibodies in mediating arthritis.
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Mod Rheumatol,
18,
429-441.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
