 |
PDBsum entry 1w8h
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Sugar binding protein
|
PDB id
|
|
|
|
1w8h
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Sugar binding protein
|
|
|
Title:
|
|
Structure of pseudomonas aeruginosa lectin ii (pa-iil)complexed with lewisa trisaccharide
|
|
Structure:
|
|
Pseudomonas aeruginosa lectin ii. Chain: a, b, c, d. Synonym: pa-iil. Engineered: yes. Other_details: each monomer contains two close calcium cations that mediate the binding of the saccharide
|
|
Source:
|
|
Pseudomonas aeruginosa. Organism_taxid: 287. Expressed in: escherichia coli. Expression_system_taxid: 469008.
|
|
Biol. unit:
|
|
Tetramer (from PDB file)
|
|
Resolution:
|
|
|
1.75Å
|
R-factor:
|
0.126
|
R-free:
|
0.169
|
|
|
Authors:
|
|
S.Perret,C.Sabin,C.Dumon,M.Budova,C.Gautier,O.Galanina,S.Ilia, N.Bovin,M.Nicaise,M.Desmadril,N.Gilboa-Garber,M.Wimmerova, E.P.Mitchell,A.Imberty
|
|
Key ref:
|
|
S.Perret
et al.
(2005).
Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa.
Biochem J,
389,
325-332.
PubMed id:
|
|
|
Date:
|
|
|
21-Sep-04
|
Release date:
|
31-Mar-05
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q9HYN5
(Q9HYN5_PSEAE) -
Fucose-binding lectin PA-IIL from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
|
|
|
|
Seq:
Struc:
|
|
|
|
115 a.a.
114 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Biochem J
389:325-332
(2005)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa.
|
|
S.Perret,
C.Sabin,
C.Dumon,
M.Pokorná,
C.Gautier,
O.Galanina,
S.Ilia,
N.Bovin,
M.Nicaise,
M.Desmadril,
N.Gilboa-Garber,
M.Wimmerová,
E.P.Mitchell,
A.Imberty.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
One of the mechanisms contributing to the protection by breast-feeding of the
newborn against enteric diseases is related to the ability of human milk
oligosaccharides to prevent the attachment of pathogenic bacteria to the
duodenual epithelium. Indeed, a variety of fucosylated oligosaccharides,
specific to human milk, form part of the innate immune system. In the present
study, we demonstrate the specific blocking of PA-IIL, a fucose-binding lectin
of the human pathogen Pseudomonas aeruginosa, by milk oligosaccharides. Two
fucosylated epitopes, Lewis a and 3-fucosyl-lactose (Lewis x glucose analogue)
bind to the lectin with dissociation constants of 2.2x10(-7) M and 3.6x10(-7) M
respectively. Thermodynamic studies indicate that these interactions are
dominated by enthalpy. The entropy contribution is slightly favourable when
binding to fucose and to the highest-affinity ligand, Lewis a. The
high-resolution X-ray structures of two complexes of PA-IIL with milk
oligosaccharides allow the precise determination of the conformation of a
trisaccharide and a pentasaccharide. The different types of interaction between
the oligosaccharides and the protein involve not only hydrogen bonding, but also
calcium- and water-bridged contacts, allowing a rationalization of the
thermodynamic data. This study provides important structural information about
compounds that could be of general application in new therapeutic strategies
against bacterial infections.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
B.Ernst,
and
J.L.Magnani
(2009).
From carbohydrate leads to glycomimetic drugs.
|
| |
Nat Rev Drug Discov,
8,
661-677.
|
|
|
|
|
|
|
C.Chemani,
A.Imberty,
S.de Bentzmann,
M.Pierre,
M.Wimmerová,
B.P.Guery,
and
K.Faure
(2009).
Role of LecA and LecB lectins in Pseudomonas aeruginosa-induced lung injury and effect of carbohydrate ligands.
|
| |
Infect Immun,
77,
2065-2075.
|
|
|
|
|
|
|
B.Lerrer,
K.D.Zinger-Yosovich,
B.Avrahami,
and
N.Gilboa-Garber
(2007).
Honey and royal jelly, like human milk, abrogate lectin-dependent infection-preceding Pseudomonas aeruginosa adhesion.
|
| |
ISME J,
1,
149-155.
|
|
|
|
|
|
|
H.Feinberg,
M.E.Taylor,
and
W.I.Weis
(2007).
Scavenger receptor C-type lectin binds to the leukocyte cell surface glycan Lewis(x) by a novel mechanism.
|
| |
J Biol Chem,
282,
17250-17258.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
J.Adam,
M.Pokorná,
C.Sabin,
E.P.Mitchell,
A.Imberty,
and
M.Wimmerová
(2007).
Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference.
|
| |
BMC Struct Biol,
7,
36.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
K.Marotte,
C.Préville,
C.Sabin,
M.Moumé-Pymbock,
A.Imberty,
and
R.Roy
(2007).
Synthesis and binding properties of divalent and trivalent clusters of the Lewis a disaccharide moiety to Pseudomonas aeruginosa lectin PA-IIL.
|
| |
Org Biomol Chem,
5,
2953-2961.
|
|
|
|
|
|
|
K.Marotte,
C.Sabin,
C.Préville,
M.Moumé-Pymbock,
M.Wimmerová,
E.P.Mitchell,
A.Imberty,
and
R.Roy
(2007).
X-ray Structures and Thermodynamics of the Interaction of PA-IIL from Pseudomonas aeruginosa with Disaccharide Derivatives.
|
| |
ChemMedChem,
2,
1328-1338.
|
|
|
PDB codes:
|
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
|
|
Links
