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PDBsum entry 1ncv
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protein Protein-protein interface(s) links
Cytokine PDB id
1ncv

 

 

 

 

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Contents
Protein chain
76 a.a. *
* Residue conservation analysis
PDB id:
1ncv
Name: Cytokine
Title: Determination cc-chemokine mcp-3, nmr, 7 structures
Structure: Monocyte chemoattractant protein 3. Chain: a, b. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: ovary. Expressed in: cricetulus griseus. Expression_system_taxid: 10029
NMR struc: 7 models
Authors: S.Meunier,J.M.Bernassau,J.C.Guillemot,P.Ferrara,H.Darbon
Key ref:
S.Meunier et al. (1997). Determination of the three-dimensional structure of CC chemokine monocyte chemoattractant protein 3 by 1H two-dimensional NMR spectroscopy. Biochemistry, 36, 4412-4422. PubMed id: 9109648 DOI: 10.1021/bi9627929
Date:
05-Feb-97     Release date:   15-Oct-97    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P80098  (CCL7_HUMAN) -  C-C motif chemokine 7 from Homo sapiens
Seq:
Struc: 		99 a.a.
76 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1021/bi9627929 Biochemistry 36:4412-4422 (1997)
PubMed id: 9109648  
 
 
Determination of the three-dimensional structure of CC chemokine monocyte chemoattractant protein 3 by 1H two-dimensional NMR spectroscopy.
S.Meunier, J.M.Bernassau, J.C.Guillemot, P.Ferrara, H.Darbon.
 
  ABSTRACT  
 
MCP-3 is a beta chemokine consisting of 76 amino acid residues. It has been described to be involved in the activation of all leukocytic cells, activation mediated by the presence of multiple binding sites on the target cells. Its three-dimensional structure has been studied by making use of two-dimensional 1H NMR spectroscopy. MCP-3 exhibits the same monomeric structure as the other chemokines, i.e., a three-stranded antiparallel beta sheet covered on one face by an alpha helix. Although it belongs to the same subfamily as RANTES (Chung et al., 1995; Faitbrother et al., 1994) and hMIP-1beta (Lodi et al., 1994), the MCP-3 dimer is folded like IL-8 with the so-called alphabeta sandwich structural motif. Structural and sequence analysis gives clear indications suggesting that the other MCP chemokines may have the same quaternary structure, contrary to the other beta chemokines.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
19441883 S.L.Deshmane, S.Kremlev, S.Amini, and B.E.Sawaya (2009).
Monocyte chemoattractant protein-1 (MCP-1): an overview.
  J Interferon Cytokine Res, 29, 313-326.  
18323622 C.Barinka, A.Prahl, and J.Lubkowski (2008).
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13).
  Acta Crystallogr D Biol Crystallogr, 64, 273-278.
PDB code: 2ra4
17644519 H.Jin, X.Shen, B.R.Baggett, X.Kong, and P.J.LiWang (2007).
The human CC chemokine MIP-1beta dimer is not competent to bind to the CCR5 receptor.
  J Biol Chem, 282, 27976-27983.  
12149255 D.M.Hoover, C.Boulegue, D.Yang, J.J.Oppenheim, K.Tucker, W.Lu, and J.Lubkowski (2002).
The structure of human macrophage inflammatory protein-3alpha /CCL20. Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins.
  J Biol Chem, 277, 37647-37654.
PDB code: 1m8a
11889129 E.S.Kuloğlu, D.R.McCaslin, J.L.Markley, and B.F.Volkman (2002).
Structural rearrangement of human lymphotactin, a C chemokine, under physiological solution conditions.
  J Biol Chem, 277, 17863-17870.  
11830659 H.Lortat-Jacob, A.Grosdidier, and A.Imberty (2002).
Structural diversity of heparan sulfate binding domains in chemokines.
  Proc Natl Acad Sci U S A, 99, 1229-1234.  
  11152129 Buyong, J.Xiong, J.Lubkowski, and R.Nussinov (2000).
Homology modeling and molecular dynamics simulations of lymphotactin.
  Protein Sci, 9, 2192-2199.  
11087354 J.Blaszczyk, E.V.Coillie, P.Proost, J.V.Damme, G.Opdenakker, G.D.Bujacz, J.M.Wang, and X.Ji (2000).
Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors.
  Biochemistry, 39, 14075-14081.
PDB code: 1esr
10727234 J.S.Laurence, C.Blanpain, J.W.Burgner, M.Parmentier, and P.J.LiWang (2000).
CC chemokine MIP-1 beta can function as a monomer and depends on Phe13 for receptor binding.
  Biochemistry, 39, 3401-3409.  
  11106181 J.T.Ashfield, T.Meyers, D.Lowne, P.G.Varley, J.R.Arnold, P.Tan, J.C.Yang, L.G.Czaplewski, T.Dudgeon, and J.Fisher (2000).
Chemical modification of a variant of human MIP-1alpha; implications for dimer structure.
  Protein Sci, 9, 2047-2053.  
10913244 K.L.Mayer, and M.J.Stone (2000).
NMR solution structure and receptor peptide binding of the CC chemokine eotaxin-2.
  Biochemistry, 39, 8382-8395.
PDB codes: 1eig 1eih
9890927 A.C.LiWang, J.J.Cao, H.Zheng, Z.Lu, S.C.Peiper, and P.J.LiWang (1999).
Dynamics study on the anti-human immunodeficiency virus chemokine viral macrophage-inflammatory protein-II (VMIP-II) reveals a fully monomeric protein.
  Biochemistry, 38, 442-453.  
  10595530 A.C.Liwang, Z.X.Wang, Y.Sun, S.C.Peiper, and P.J.Liwang (1999).
The solution structure of the anti-HIV chemokine vMIP-II.
  Protein Sci, 8, 2270-2280.
PDB code: 1vmp
10379912 E.Van Coillie, J.Van Damme, and G.Opdenakker (1999).
The MCP/eotaxin subfamily of CC chemokines.
  Cytokine Growth Factor Rev, 10, 61-86.  
10320325 H.Sticht, S.E.Escher, K.Schweimer, W.G.Forssmann, P.Rösch, and K.Adermann (1999).
Solution structure of the human CC chemokine 2: A monomeric representative of the CC chemokine subtype.
  Biochemistry, 38, 5995-6002.
PDB code: 2hcc
10347159 L.G.Czaplewski, J.McKeating, C.J.Craven, L.D.Higgins, V.Appay, A.Brown, T.Dudgeon, L.A.Howard, T.Meyers, J.Owen, S.R.Palan, P.Tan, G.Wilson, N.R.Woods, C.M.Heyworth, B.I.Lord, D.Brotherton, R.Christison, S.Craig, S.Cribbes, R.M.Edwards, S.J.Evans, R.Gilbert, P.Morgan, E.Randle, N.Schofield, P.G.Varley, J.Fisher, J.P.Waltho, and M.G.Hunter (1999).
Identification of amino acid residues critical for aggregation of human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES. Characterization of active disaggregated chemokine variants.
  J Biol Chem, 274, 16077-16084.
PDB codes: 1b50 1b53
9649315 J.S.Laurence, A.C.LiWang, and P.J.LiWang (1998).
Effect of N-terminal truncation and solution conditions on chemokine dimer stability: nuclear magnetic resonance structural analysis of macrophage inflammatory protein 1 beta mutants.
  Biochemistry, 37, 9346-9354.  
9622482 W.Shao, L.F.Jerva, J.West, E.Lolis, and B.I.Schweitzer (1998).
Solution structure of murine macrophage inflammatory protein-2.
  Biochemistry, 37, 8303-8313.
PDB code: 1mi2
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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