› 7f1n

X-ray diffraction
2.14Å resolution

Beta-Glucosidase

Released:
DOI: 10.2210/pdb7f1n/pdb
PDB entry 7f1n coloured by chain, front view.
PDB entry 7f1n coloured by chain, side view.
PDB entry 7f1n coloured by chain, top view.
Source organism: Thermofilum sp. ex4484_79
Primary publication:
Structural and Catalytic Characterization of TsBGL, a β-Glucosidase From Thermofilum sp. ex4484_79.
Chen A, Wang D, Ji R, Li J, Gu S, Tang R, Ji C
Front Microbiol 12 723678 (2021)
PMID: 34659150

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 511 amino acids
Theoretical weight: 58.72 KDa
Source organism: Thermofilum sp. ex4484_79
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A256XQM7 (Residues: 1-511; Coverage: 100%)
Gene name: B6U94_07015
Sequence domains: Glycosyl hydrolase family 1

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 76.72Å b: 62.21Å c: 112.45Å
α: 90° β: 98.508° γ: 90°
R-values:
R R work R free
0.212 0.21 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Inactivation and process intensification of β-glucosidase in biomass utilization.
Mu et al. (2023)
3 other articles

1 mention without citation

Structural and Catalytic Characterization of TsBGL, a β-Glucosidase From Thermofilum sp. ex4484_79.
Chen et al. (2021)