› 7ztl

X-ray diffraction
1.9Å resolution

Crystal structure of a covalently linked Aurora-A N-Myc complex

Released:
DOI: 10.2210/pdb7ztl/pdb
PDB entry 7ztl coloured by chain, front view.
PDB entry 7ztl coloured by chain, side view.
PDB entry 7ztl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a covalently linked Aurora-A-MYCN complex.
Diebold M, Schönemann L, Eilers M, Sotriffer C, Schindelin H
Acta Crystallogr D Struct Biol 79 1-9 (2023)
PMID: 36601802

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127233 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase A Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
N-myc proto-oncogene protein Chain: B
Molecule details ›
Chain: B
Length: 62 amino acids
Theoretical weight: 7.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04198 (Residues: 28-89; Coverage: 13%)
Gene names: BHLHE37, MYCN, NMYC

Ligands and Environments

6 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand GOL 3 x GOL
Ligand BCN 1 x BCN
Ligand NA 1 x NA
Ligand JWG 1 x JWG
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P3221
Unit cell:
a: 86.614Å b: 86.614Å c: 91.789Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.163 0.193
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

Computational completion of the Aurora interaction region of N-Myc in the Aurora a kinase complex.
Altiner et al. (2023)
1 more