› 7ktr

Electron Microscopy
2.93Å resolution

Cryo-EM structure of the human SAGA coactivator complex (TRRAP, core)

Released:
DOI: 10.2210/pdb7ktr/pdb
PDB entry 7ktr coloured by chain, front view.
PDB entry 7ktr coloured by chain, side view.
PDB entry 7ktr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human SAGA coactivator complex.
Herbst DA, Esbin MN, Louder RK, Dugast-Darzacq C, Dailey GM, Fang Q, Darzacq X, Tjian R, Nogales E
Nat Struct Mol Biol 28 989-996 (2021)
PMID: 34811519
Related structures: EMD-23027

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-127400 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Transformation/transcription domain-associated protein Chain: A
Molecule details ›
Chain: A
Length: 3195 amino acids
Theoretical weight: 353.17 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y4A5 (Residues: 369-3287, 3288-3859; Coverage: 82%)
Gene names: PAF400, TRRAP
Sequence domains:
TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L Chain: B
Molecule details ›
Chain: B
Length: 589 amino acids
Theoretical weight: 66.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75529 (Residues: 1-589; Coverage: 100%)
Gene names: PAF65B, TAF5L
Sequence domains:
Transcription factor SPT20 homolog Chain: C
Molecule details ›
Chain: C
Length: 811 amino acids
Theoretical weight: 88.13 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q8NEM7 (Residues: 1-531, 532-728; Coverage: 94%)
Gene names: C13orf19, FAM48A, FP757, SUPT20H
Sequence domains: Spt20, SEP domain
STAGA complex 65 subunit gamma Chain: D
Molecule details ›
Chain: D
Length: 704 amino acids
Theoretical weight: 78.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O94864 (Residues: 51-414; Coverage: 86%)
Gene names: KIAA0764, SUPT7L
Sequence domains: Bromodomain associated
Transcription initiation factor TFIID subunit 9B Chain: E
Molecule details ›
Chain: E
Length: 251 amino acids
Theoretical weight: 27.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9HBM6 (Residues: 1-251; Coverage: 100%)
Gene names: TAF9B, TAF9L
Sequence domains: Transcription initiation factor IID, 31kD subunit
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L Chain: F
Molecule details ›
Chain: F
Length: 622 amino acids
Theoretical weight: 67.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y6J9 (Residues: 1-622; Coverage: 100%)
Gene names: PAF65A, TAF6L
Sequence domains:
Transcription initiation factor TFIID subunit 12 Chain: G
Molecule details ›
Chain: G
Length: 161 amino acids
Theoretical weight: 17.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q16514 (Residues: 1-161; Coverage: 100%)
Gene names: TAF12, TAF15, TAF2J, TAFII20
Sequence domains: Transcription initiation factor TFIID subunit A
Transcription initiation factor TFIID subunit 10 Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q12962 (Residues: 1-218; Coverage: 100%)
Gene names: TAF10, TAF2A, TAF2H, TAFII30
Sequence domains: Transcription initiation factor TFIID 23-30kDa subunit
Transcription initiation protein SPT3 homolog Chain: I
Molecule details ›
Chain: I
Length: 299 amino acids
Theoretical weight: 33.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O75486 (Residues: 1-317; Coverage: 94%)
Gene names: SPT3, SUPT3H
Sequence domains: Transcription initiation factor IID, 18kD subunit
Transcriptional adapter 1 Chain: J
Molecule details ›
Chain: J
Length: 335 amino acids
Theoretical weight: 37.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q96BN2 (Residues: 1-335; Coverage: 100%)
Gene names: TADA1, TADA1L
Sequence domains: Transcriptional regulator of RNA polII, SAGA, subunit
Ataxin-7 Chain: N
Molecule details ›
Chain: N
Length: 892 amino acids
Theoretical weight: 95.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O15265 (Residues: 1-892; Coverage: 100%)
Gene names: ATXN7, SCA7
Sequence domains: SCA7, zinc-binding domain

Ligands and Environments

1 bound ligand:
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.93Å
Relevant EMDB volumes: EMD-23027
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Assembly of RNA polymerase II transcription initiation complexes.
Farnung et al. (2022)
4 more

1 mention without citation

Structure of the human SAGA coactivator complex.
Herbst et al. (2021)