› 7eq1

Electron Microscopy
3.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209132 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.88 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04896 (Residues: 1-3, 27-67, 205-251, 266-394; Coverage: 56%)
  • Canonical: P63097 (Residues: 4-19, 61-181, 229-242; Coverage: 40%)
Gene names: GNAI1, GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Adhesion G-protein coupled receptor G5 Chain: R
Molecule details ›
Chain: R
Length: 302 amino acids
Theoretical weight: 33.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8IZF4 (Residues: 227-528; Coverage: 60%)
Gene names: ADGRG5, GPR114, PGR27, UNQ2524/PRO6017
Sequence domains: 7 transmembrane receptor (Secretin family)
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 247 amino acids
Theoretical weight: 26.28 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda ascovirus 1a
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: Y
Molecule details ›
Chain: Y
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-31254
Expression systems:
  • Spodoptera frugiperda
  • Spodoptera frugiperda ascovirus 1a

Quick links

Citations

6 review citations

Adhesion G protein-coupled receptor gluing action guides tissue development and disease.
Sreepada et al. (2022)
5 more