› 7a5e

X-ray diffraction
1.9Å resolution

OSM-3 kinesin motor domain complexed with Mg.AMPPNP

Released:
DOI: 10.2210/pdb7a5e/pdb
PDB entry 7a5e coloured by chain, front view.
PDB entry 7a5e coloured by chain, side view.
PDB entry 7a5e coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism.
Varela PF, Chenon M, Velours C, Verhey KJ, Ménétrey J, Gigant B
FEBS Open Bio 11 564-577 (2021)
PMID: 33513284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Osmotic avoidance abnormal protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 38.22 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P46873 (Residues: 2-337; Coverage: 48%)
Gene names: M02B7.3, osm-3
Sequence domains: Kinesin motor domain

Ligands and Environments

4 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
Ligand SO4 4 x SO4
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 100.03Å b: 102.2Å c: 140.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.231
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

A kinesin-1 variant reveals motor-induced microtubule damage in cells.
Budaitis et al. (2022)

1 mention without citation

Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism.
Varela et al. (2021)