Pathways & interactions
Kinesin motor domain superfamily (IPR036961)
Short name: Kinesin_motor_dom_sf
- Myosin head, motor domain (IPR001609)
- Kinesin motor domain (IPR001752)
- Kinesin-like protein KIF22 (Kid) (IPR026986)
- Kinesin-like protein KIFC3 (IPR027325)
- Kinesin-like protein KIF20A (IPR027326)
- P-loop containing nucleoside triphosphate hydrolase (IPR027417)
- Kinesin-like protein (IPR027640)
- Unconventional myosin-IXb (IPR028557)
- Kinesin-like protein KIF12 (IPR028763)
- Kinesin-like protein KIF20B (IPR028828)
- Kinesin-like protein KIN-14 (IPR030109)
- Spindle pole body-associated protein Vik1/Cik1, microtubule binding domain (IPR031852)
- Kinesin-like protein KIF23 (IPR032924)
- Armadillo repeat-containing kinesin-like protein, plants (IPR033291)
- Unconventional myosin-XV (IPR035487)
- Class II myosin, Myh3, motor domain (IPR036000)
- Plant myosin, class XI, motor domain (IPR036018)
- Plant myosin class VIII, motor domain (IPR036022)
- Class IX myosin, motor domain (IPR036023)
- Class XX myosin, motor domain (IPR036029)
- Class XIX myosin, motor domain (IPR036035)
- Class XIV myosin, motor domain (IPR036044)
- Class XV myosin, motor domain (IPR036057)
- Class XVIII myosin, motor domain (IPR036064)
- Class I myosin, motor domain (IPR036072)
- Class III myosin, motor domain (IPR036083)
- Class V myosin, motor domain (IPR036103)
- Class VII myosin, motor domain (IPR036106)
- Class VI myosin, motor domain (IPR036114)
- Class X myosin, motor domain (IPR036124)
- Class XXI myosin, motor domain (IPR036125)
Kinesin [PMID: 8542443, PMID: 2142876, PMID: 14732151] is a microtubule-associated force-producing protein that may play a role in organelle transport. The kinesin motor activity is directed toward the microtubule's plus end. Kinesin is an oligomeric complex composed of two heavy chains and two light chains. The maintenance of the quaternary structure does not require interchain disulphide bonds.
The heavy chain is composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it is known to hydrolyse ATP, to bind and move on microtubules), a central alpha-helical coiled coil domain that mediates the heavy chain dimerisation; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
The kinesin motor domain comprises five motifs, namely N1 (P-loop), N2 (Switch I), N3 (Switch II), N4 and L2 (KVD finger) [PMID: 20587735]. It has a mixed eight stranded beta-sheet core with flanking solvent exposed alpha-helices and a small three-stranded antiparallel beta-sheet in the N-terminal region [PMID: 15236970].
- Drosophila melanogaster claret segregational protein (ncd). Ncd is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The ncd motor activity is directed toward the microtubule's minus end.
- Homo sapiens CENP-E [PMID: 1832505]. CENP-E is a protein that associates with kinetochores during chromosome congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. CENP-E is probably an important motor molecule in chromosome movement and/or spindle elongation.
- H. sapiens mitotic kinesin-like protein-1 (MKLP-1), a motor protein whose activity is directed toward the microtubule's plus end.
- Saccharomyces cerevisiae KAR3 protein, which is essential for nuclear fusion during mating. KAR3 may mediate microtubule sliding during nuclear fusion and possibly mitosis.
- S. cerevisiae CIN8 and KIP1 proteins which are required for the assembly of the mitotic spindle. Both proteins seem to interact with spindle microtubules to produce an outwardly directed force acting upon the poles.
- Emericella nidulans (Aspergillus nidulans) bimC, which plays an important role in nuclear division.
- A. nidulans klpA.
- Caenorhabditis elegans unc-104, which may be required for the transport of substances needed for neuronal cell differentiation.
- C. elegans osm-3.
- Xenopus laevis Eg5, which may be involved in mitosis.
- Arabidopsis thaliana KatA, KatB and katC.
- Chlamydomonas reinhardtii FLA10/KHP1 and KLP1. Both proteins seem to play a role in the rotation or twisting of the microtubules of the flagella.
- C. elegans hypothetical protein T09A5.2.
The kinesin motor domain is located in the N-terminal part of most of the above proteins, with the exception of KAR3, klpA, and ncd where it is located in the C-terminal section.
The kinesin motor domain contains about 330 amino acids. An ATP-binding motif of type A is found near position 80 to 90, the C-terminal half of the domain is involved in microtubule-binding.
Interestingly, kinesin motor domain has a striking structural similarity to the core of the catalytic domain of the actin-based motor myosin [PMID: 8606779].
- G3DSA:3.40.850.10 (G3DSA:3.40.850.10)