6aon

X-ray diffraction
1.72Å resolution

1.72 Angstrom Resolution Crystal Structure of 2-Oxoglutarate Dehydrogenase Complex Subunit Dihydrolipoamide Dehydrogenase from Bordetella pertussis in Complex with FAD

Released:
Entry authors: Minasov G, Wawrzak Z, Skarina T, McChesney C, Grimshaw S, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 50.44 KDa
Source organism: Bordetella pertussis Tohama I
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7VZ16 (Residues: 1-475; Coverage: 100%)
Gene names: BP1126, odhL
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P41
Unit cell:
a: 68.801Å b: 68.801Å c: 199.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.205
Expression system: Escherichia coli BL21(DE3)