› 5yei

X-ray diffraction
2.3Å resolution

Mechanistic insight into the regulation of Pseudomonas aeruginosa aspartate kinase

Released:
DOI: 10.2210/pdb5yei/pdb
PDB entry 5yei coloured by chain, front view.
PDB entry 5yei coloured by chain, side view.
PDB entry 5yei coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Mechanistic insights into the allosteric regulation of Pseudomonas aeruginosa aspartate kinase.
Li CC, Yang MJ, Liu L, Li T, Peng CT, He LH, Song YJ, Zhu YB, Shen YL, Yang J, Zhao NL, Zhao C, Zhou QX, Li H, Kang M, Tong AP, Tang H, Bao R
Biochem J 475 1107-1119 (2018)
PMID: 29382741

Function and Biology Details

Reaction catalysed: 
ATP + L-aspartate = ADP + 4-phospho-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130643 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartokinase Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 164 amino acids
Theoretical weight: 17.75 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O69077 (Residues: 249-412; Coverage: 40%)
Gene names: PA0904, lysC
Sequence domains:
Structure domains: Alpha-Beta Plaits
Aspartokinase Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 412 amino acids
Theoretical weight: 44.44 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O69077 (Residues: 1-412; Coverage: 100%)
Gene names: PA0904, lysC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand THR 8 x THR
Ligand LYS 4 x LYS
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 82.459Å b: 171.793Å c: 88.932Å
α: 90° β: 90.29° γ: 90°
R-values:
R R work R free
0.224 0.222 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Construction of Novel Aspartokinase Mutant A380I and Its Characterization by Molecular Dynamics Simulation.
Han et al. (2018)
3 more