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CH3 chimera of human 14-3-3 sigma with the StARD1 peptide including Ser57

X-ray diffraction
3.9Å resolution
PDB entry 5oma coloured by chain, front view.
PDB entry 5oma coloured by chain, side view.
PDB entry 5oma coloured by chain, top view.
Source organism: Homo sapiens
Assemblies composition:
hetero trimer (preferred)
homo dimer
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: 14-3-3 protein sigma, Steroidogenic acute regulatory protein, mitochondrial and peptide
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Multimeric state: hetero trimer
Accessible surface area: 23196.05 Å2
Buried surface area: 2261.85 Å2
Dissociation area: 39.7 Å2
Dissociation energy (ΔGdiss): -1.81 kcal/mol
Dissociation entropy (TΔSdiss): 3.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-152121
Assembly 2
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Assembly Name: 14-3-3 protein sigma, Steroidogenic acute regulatory protein, mitochondrial
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Multimeric state: homo dimer
Accessible surface area: 23267.46 Å2
Buried surface area: 2487.6 Å2
Dissociation area: 1,047.46 Å2
Dissociation energy (ΔGdiss): -0.27 kcal/mol
Dissociation entropy (TΔSdiss): 13.07 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-152120

Macromolecules

14-3-3 protein sigma; Steroidogenic acute regulatory protein, mitochondrial
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 27.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
  • Canonical: P49675 (Residues: 54-62; Coverage: 3%)
    • nullnull
Gene names: HME1, SFN, STAR, STARD1
Pfam: 14-3-3 protein
InterPro:
CATH: 14-3-3 domain
14-3-3 protein sigma in PDB entry 5oma, assembly 1, front view.
14-3-3 protein sigma in PDB entry 5oma, assembly 1, side view.
14-3-3 protein sigma in PDB entry 5oma, assembly 1, top view.
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Undetermined peptide
Chain: H
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Undetermined peptide in PDB entry 5oma, assembly 1, front view.
Undetermined peptide in PDB entry 5oma, assembly 1, side view.
Undetermined peptide in PDB entry 5oma, assembly 1, top view.
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