› 5nly

X-ray diffraction
2Å resolution

Brag2 Sec7-PH (390-763), P212121

Released:
DOI: 10.2210/pdb5nly/pdb
PDB entry 5nly coloured by chain, front view.
PDB entry 5nly coloured by chain, side view.
PDB entry 5nly coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple interactions between an Arf/GEF complex and charged lipids determine activation kinetics on the membrane.
Karandur D, Nawrotek A, Kuriyan J, Cherfils J
Proc Natl Acad Sci U S A 114 11416-11421 (2017)
PMID: 28923919

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179522 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IQ motif and SEC7 domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 47.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DN90 (Residues: 512-885; Coverage: 39%)
Gene names: ARFGEP100, BRAG2, IQSEC1, KIAA0763
Sequence domains:
IQ motif and SEC7 domain-containing protein 1 Chain: B
Molecule details ›
Chain: B
Length: 405 amino acids
Theoretical weight: 47.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DN90 (Residues: 512-885; Coverage: 39%)
Gene names: ARFGEP100, BRAG2, IQSEC1, KIAA0763
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand CME 9 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 63.893Å b: 66.039Å c: 206.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.233
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Computational Modeling of Realistic Cell Membranes.
Marrink et al. (2019)
5 more

1 mention without citation

Multiple interactions between an Arf/GEF complex and charged lipids determine activation kinetics on the membrane.
Karandur et al. (2017)