› 4r1t

X-ray diffraction
1.7Å resolution

Crystal structure of Petunia hydrida cinnamoyl-CoA reductase

Released:
DOI: 10.2210/pdb4r1t/pdb
PDB entry 4r1t coloured by chain, front view.
PDB entry 4r1t coloured by chain, side view.
PDB entry 4r1t coloured by chain, top view.
Source organism: Petunia x hybrida
Primary publication:
Structural studies of cinnamoyl-CoA reductase and cinnamyl-alcohol dehydrogenase, key enzymes of monolignol biosynthesis.
Pan H, Zhou R, Louie GV, Mühlemann JK, Bomati EK, Bowman ME, Dudareva N, Dixon RA, Noel JP, Wang X
Plant Cell 26 3709-27 (2014)
PMID: 25217505

Function and Biology Details

Reaction catalysed: 
Cinnamaldehyde + CoA + NADP(+) = cinnamoyl-CoA + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100261 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cinnamoyl-CoA reductase 1 Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 37.27 KDa
Source organism: Petunia x hybrida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A059TC02 (Residues: 1-333; Coverage: 100%)
Gene name: CCR1
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand I2I 1 x I2I
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6122
Unit cell:
a: 127.844Å b: 127.844Å c: 80.016Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

36 citation in other articles

How Embryophytic is the Biosynthesis of Phenylpropanoids and their Derivatives in Streptophyte Algae?
de Vries et al. (2017)
35 more