› 4q7a

X-ray diffraction
2.05Å resolution

Crystal Structure of N-acetyl-ornithine/N-acetyl-lysine Deacetylase from Sphaerobacter thermophilus

Released:
DOI: 10.2210/pdb4q7a/pdb
PDB 4q7a coloured by chain and viewed from the front.
PDB 4q7a coloured by chain and viewed from the side.
PDB 4q7a coloured by chain and viewed from the top.
Source organism: Sphaerobacter thermophilus DSM 20745
Entry authors: Kim Y, Tesar C, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative [LysW]-lysine hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 39.1 KDa
Source organism: Sphaerobacter thermophilus DSM 20745
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1C6B5 (Residues: 1-367; Coverage: 100%)
Gene names: Sthe_0214, lysK
Sequence domains: Peptidase family M20/M25/M40
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P42
Unit cell:
a: 119.54Å b: 119.54Å c: 119.212Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.171
Expression system: Escherichia coli BL21(DE3)

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