4q7a

X-ray diffraction
2.05Å resolution

Crystal Structure of N-acetyl-ornithine/N-acetyl-lysine Deacetylase from Sphaerobacter thermophilus

Released:
Entry authors: Kim Y, Tesar C, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative [LysW]-lysine hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 39.1 KDa
Source organism: Sphaerobacter thermophilus DSM 20745
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1C6B5 (Residues: 1-367; Coverage: 100%)
Gene names: Sthe_0214, lysK
Sequence domains: Peptidase family M20/M25/M40
Structure domains:

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P42
Unit cell:
a: 119.54Å b: 119.54Å c: 119.212Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.171
Expression system: Escherichia coli BL21(DE3)