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Crystal structure of CobT E174A complexed with adenine

X-ray diffraction
1.9Å resolution
PDB entry 4kqf coloured by chain, front view.
PDB entry 4kqf coloured by chain, side view.
PDB entry 4kqf coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
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Multimeric state: homo dimer
Accessible surface area: 21289.44 Å2
Buried surface area: 6145.6 Å2
Dissociation area: 2,075.12 Å2
Dissociation energy (ΔGdiss): 26.39 kcal/mol
Dissociation entropy (TΔSdiss): 13.91 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-170230

Macromolecules

Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Chain: A
Length: 356 amino acids
Theoretical weight: 36.59 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05603 (Residues: 1-356; Coverage: 100%)
Gene names: STM2016, cobT
Pfam: Phosphoribosyltransferase
InterPro:
CATH:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase in PDB entry 4kqf, assembly 1, front view.
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase in PDB entry 4kqf, assembly 1, side view.
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase in PDB entry 4kqf, assembly 1, top view.
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