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DNA polymerase beta matched substrate complex with Ca2+, 0 s

X-ray diffraction
1.916Å resolution
PDB entry 4kld coloured by chain, front view.
PDB entry 4kld coloured by chain, side view.
PDB entry 4kld coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
3 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA polymerase beta and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 20478.39 Å2
Buried surface area: 6232.26 Å2
Dissociation area: 964.6 Å2
Dissociation energy (ΔGdiss): 26.72 kcal/mol
Dissociation entropy (TΔSdiss): 9.8 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-117743

Macromolecules

DNA polymerase beta
Chain: A
Length: 335 amino acids
Theoretical weight: 38.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 1-335; Coverage: 100%)
Gene name: POLB
Pfam:
InterPro:
CATH:
DNA polymerase beta in PDB entry 4kld, assembly 1, front view.
DNA polymerase beta in PDB entry 4kld, assembly 1, side view.
DNA polymerase beta in PDB entry 4kld, assembly 1, top view.
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DNA chain T
Name: 5'-D(*CP*CP*GP*AP*CP*GP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3'
Representative chains: T
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
5'-D(*CP*CP*GP*AP*CP*GP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3' in PDB entry 4kld, assembly 1, front view.
5'-D(*CP*CP*GP*AP*CP*GP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3' in PDB entry 4kld, assembly 1, side view.
5'-D(*CP*CP*GP*AP*CP*GP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3' in PDB entry 4kld, assembly 1, top view.
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DNA chain P
Name: 5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3'
Representative chains: P
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3' in PDB entry 4kld, assembly 1, front view.
5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3' in PDB entry 4kld, assembly 1, side view.
5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*C)-3' in PDB entry 4kld, assembly 1, top view.
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DNA chain D
Name: 5'-D(P*GP*TP*CP*GP*G)-3'
Representative chains: D
Source organism: Homo sapiens [9606]
Expression system: Not provided
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
5'-D(P*GP*TP*CP*GP*G)-3' in PDB entry 4kld, assembly 1, front view.
5'-D(P*GP*TP*CP*GP*G)-3' in PDB entry 4kld, assembly 1, side view.
5'-D(P*GP*TP*CP*GP*G)-3' in PDB entry 4kld, assembly 1, top view.
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