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Y-family DNA polymerase chimera Dbh-Dpo4-Dpo4 #1

X-ray diffraction
1.898Å resolution
PDB entry 4f4w coloured by chain, front view.
PDB entry 4f4w coloured by chain, side view.
PDB entry 4f4w coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA polymerase IV and DNA
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Multimeric state: hetero trimer
Accessible surface area: 19695.17 Å2
Buried surface area: 5186.55 Å2
Dissociation area: 1,172.12 Å2
Dissociation energy (ΔGdiss): 33.88 kcal/mol
Dissociation entropy (TΔSdiss): 10.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-238519
Assembly 2
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Assembly Name: DNA polymerase IV and DNA
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Multimeric state: hetero trimer
Accessible surface area: 19866.71 Å2
Buried surface area: 5127.28 Å2
Dissociation area: 1,168.36 Å2
Dissociation energy (ΔGdiss): 32.71 kcal/mol
Dissociation entropy (TΔSdiss): 10.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-238519

Macromolecules

DNA polymerase IV
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.12 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q4JB80 (Residues: 1-231; Coverage: 65%)
  • Canonical: Q97W02 (Residues: 231-352; Coverage: 35%)
    • nullnull
Gene names: SSO2448, Saci_0554, dbh, dpo4
Pfam:
InterPro:
CATH:
DNA polymerase IV in PDB entry 4f4w, assembly 1, front view.
DNA polymerase IV in PDB entry 4f4w, assembly 1, side view.
DNA polymerase IV in PDB entry 4f4w, assembly 1, top view.
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DNA chains E, P
Name: DNA (5'-D(*GP*GP*CP*AP*CP*TP*GP*AP*TP*CP*GP*GP*C)-3')
Representative chains: E, P
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
DNA (5'-D(*GP*GP*CP*AP*CP*TP*GP*AP*TP*CP*GP*GP*C)-3') in PDB entry 4f4w, assembly 1, front view.
DNA (5'-D(*GP*GP*CP*AP*CP*TP*GP*AP*TP*CP*GP*GP*C)-3') in PDB entry 4f4w, assembly 1, side view.
DNA (5'-D(*GP*GP*CP*AP*CP*TP*GP*AP*TP*CP*GP*GP*C)-3') in PDB entry 4f4w, assembly 1, top view.
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DNA chains F, T
Name: DNA (5'-D(*TP*TP*AP*CP*GP*GP*CP*CP*GP*AP*TP*CP*AP*GP*TP*GP*CP*C)-3')
Representative chains: F, T
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
DNA (5'-D(*TP*TP*AP*CP*GP*GP*CP*CP*GP*AP*TP*CP*AP*GP*TP*GP*CP*C)-3') in PDB entry 4f4w, assembly 1, front view.
DNA (5'-D(*TP*TP*AP*CP*GP*GP*CP*CP*GP*AP*TP*CP*AP*GP*TP*GP*CP*C)-3') in PDB entry 4f4w, assembly 1, side view.
DNA (5'-D(*TP*TP*AP*CP*GP*GP*CP*CP*GP*AP*TP*CP*AP*GP*TP*GP*CP*C)-3') in PDB entry 4f4w, assembly 1, top view.
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