4c4n

X-ray diffraction
2.36Å resolution

Crystal structure of the Sonic Hedgehog-heparin complex

Released:
Source organism: Mus musculus
Primary publication:
Structural insights into proteoglycan-shaped Hedgehog signaling.
Proc Natl Acad Sci U S A 110 16420-5 (2013)
PMID: 24062467

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-178922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Sonic hedgehog protein N-product Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62226 (Residues: 40-195; Coverage: 38%)
Gene names: Hhg1, Shh
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

Carbohydrate polymer : NEW Components: IDS, SGN
Carbohydrate polymer : NEW Components: IDS, SGN
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 95.68Å b: 61.6Å c: 62.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.232
Expression system: Escherichia coli BL21(DE3)