› 3u4a

X-ray diffraction
2.2Å resolution

From soil to structure: a novel dimeric family 3-beta-glucosidase isolated from compost using metagenomic analysis

Released:
DOI: 10.2210/pdb3u4a/pdb
PDB entry 3u4a coloured by chain, front view.
PDB entry 3u4a coloured by chain, side view.
PDB entry 3u4a coloured by chain, top view.
Source organism: compost metagenome
Entry authors: McAndrew RP, Park JI, Reindl W, Friedland GD, D'haeseleer P, Northen T, Sale KL, Simmons BA, Adams PD

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
JMB19063 Chains: A, B
Molecule details ›
Chains: A, B
Length: 775 amino acids
Theoretical weight: 85.48 KDa
Source organism: compost metagenome
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand BGC 2 x BGC
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 121.748Å b: 121.748Å c: 242.816Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.222
Expression system: Escherichia coli

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