2x0n

X-ray diffraction
3.2Å resolution

Structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei determined from Laue data

Released:

Function and Biology Details

Reaction catalysed:
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase, glycosomal Chains: A, B, O, P, Q, R
Molecule details ›
Chains: A, B, O, P, Q, R
Length: 359 amino acids
Theoretical weight: 39.09 KDa
Source organism: Trypanosoma brucei brucei
UniProt:
  • Canonical: P22512 (Residues: 1-359; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 6 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.7
Spacegroup: P21212
Unit cell:
a: 135.52Å b: 256.27Å c: 114.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.15 0.218