› 2gzm

X-ray diffraction
1.99Å resolution

Crystal Structure of the Glutamate Racemase from Bacillus anthracis

Released:
DOI: 10.2210/pdb2gzm/pdb
PDB entry 2gzm coloured by chain, front view.
PDB entry 2gzm coloured by chain, side view.
PDB entry 2gzm coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.
May M, Mehboob S, Mulhearn DC, Wang Z, Yu H, Thatcher GR, Santarsiero BD, Johnson ME, Mesecar AD
J Mol Biol 371 1219-37 (2007)
PMID: 17610893

Function and Biology Details

Reaction catalysed: 
L-glutamate = D-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-105743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate racemase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 29.68 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8PKK6 (Residues: 2-268; Coverage: 99%)
Gene names: GBAA_4717, murI, racE2
Sequence domains: Asp/Glu/Hydantoin racemase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand DGL 4 x DGL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 56.346Å b: 90.518Å c: 116.799Å
α: 90° β: 100.021° γ: 90°
R-values:
R R work R free
0.203 0.203 0.241
Expression system: Escherichia coli

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Citations

6 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
5 more

4 mentions without citation

On the functional significance of soft modes predicted by coarse-grained models for membrane proteins.
Bahar I. (2010)
3 more