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tRNA-guanine Transglycosylase (TGT) in complex with 6-Amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one

X-ray diffraction
1.58Å resolution
PDB entry 1y5w coloured by chain, front view.
PDB entry 1y5w coloured by chain, side view.
PDB entry 1y5w coloured by chain, top view.
Source organism: Zymomonas mobilis
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Queuine tRNA-ribosyltransferase
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Multimeric state: homo dimer
Accessible surface area: 24749.33 Å2
Buried surface area: 5696.76 Å2
Dissociation area: 1,624.29 Å2
Dissociation energy (ΔGdiss): 10.13 kcal/mol
Dissociation entropy (TΔSdiss): 13.9 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-151274

Macromolecules

Queuine tRNA-ribosyltransferase
Chain: A
Length: 385 amino acids
Theoretical weight: 42.79 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28720 (Residues: 2-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Pfam: Queuine tRNA-ribosyltransferase
InterPro:
CATH: Queuine tRNA-ribosyltransferase-like
SCOP: tRNA-guanine transglycosylase
Queuine tRNA-ribosyltransferase in PDB entry 1y5w, assembly 1, front view.
Queuine tRNA-ribosyltransferase in PDB entry 1y5w, assembly 1, side view.
Queuine tRNA-ribosyltransferase in PDB entry 1y5w, assembly 1, top view.
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