› 6k65

X-ray diffraction
1.65Å resolution

Application of anti-helix antibodies in protein structure determination (9014-1P4B)

Released:
DOI: 10.2210/pdb6k65/pdb
PDB entry 6k65 coloured by chain, front view.
PDB entry 6k65 coloured by chain, side view.
PDB entry 6k65 coloured by chain, top view.
Source organisms:
Primary publication:
Application of antihelix antibodies in protein structure determination.
Kim JW, Kim S, Lee H, Cho G, Kim SC, Lee H, Jin MS, Lee JO
Proc Natl Acad Sci U S A 116 17786-17791 (2019)
PMID: 31371498

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208866 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin G-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 8.88 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli
UniProt:
  • Canonical: P02976 (Residues: 218-266; Coverage: 10%)
Gene names: SAOUHSC_00069, spa
Sequence domains: B domain
1P4B variable light chain Chain: L
Molecule details ›
Chain: L
Length: 115 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
1P4B variable heavy chain Chain: H
Molecule details ›
Chain: H
Length: 113 amino acids
Theoretical weight: 12.22 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21212
Unit cell:
a: 35.62Å b: 120.181Å c: 84.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.192
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.
Mukherjee et al. (2020)
7 more

1 mention without citation

Application of antihelix antibodies in protein structure determination.
Kim et al. (2019)