5s5v

X-ray diffraction
2.7Å resolution

Tubulin-Z32386228-complex

Released:
Primary publication:
Comprehensive Analysis of Binding Sites in Tubulin.
OpenAccess logo Angew Chem Int Ed Engl 60 13331-13342 (2021)
PMID: 33951246

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-237127 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Tubulin-Tyrosine Ligase Chain: F
Molecule details ›
Chain: F
Length: 384 amino acids
Theoretical weight: 44.38 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 105.23Å b: 159.54Å c: 179.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.267
Expression system: Escherichia coli