› 4idg

X-ray diffraction
1.9Å resolution

Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (TARGET EFI-506441) with bound NAD, monoclinic form 2

Released:
DOI: 10.2210/pdb4idg/pdb
PDB entry 4idg coloured by chain, front view.
PDB entry 4idg coloured by chain, side view.
PDB entry 4idg coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Vetting MW, Groninger-Poe F, Morisco LL, Wasserman SR, Sojitra S, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.08 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9CL58 (Residues: 1-327; Coverage: 100%)
Gene names: Atu5463, galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 3 x CL
Ligand NAD 2 x NAD
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 77.388Å b: 57.703Å c: 95.418Å
α: 90° β: 108.68° γ: 90°
R-values:
R R work R free
0.179 0.177 0.216
Expression system: Escherichia coli BL21(DE3)

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