› 4hn8

X-ray diffraction
2.2Å resolution

Crystal structure of a putative D-glucarate dehydratase from Pseudomonas mendocina ymp

Released:
DOI: 10.2210/pdb4hn8/pdb
PDB entry 4hn8 coloured by chain, front view.
PDB entry 4hn8 coloured by chain, side view.
PDB entry 4hn8 coloured by chain, top view.
Source organism: Pseudomonas mendocina ymp
Entry authors: Hegde RP, Toro R, Burley SK, Almo SC, Ramagopal UA, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glucarate dehydratase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 473 amino acids
Theoretical weight: 52.88 KDa
Source organism: Pseudomonas mendocina ymp
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4XRL3 (Residues: 2-463; Coverage: 100%)
Gene name: Pmen_1214
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 9 x GOL
1 modified residue:
Ligand MSE 120 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 126.355Å b: 148.835Å c: 198.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.222
Expression system: Escherichia coli BL21(DE3)

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