› 3q1e

X-ray diffraction
1.95Å resolution

Crystal structure of Y116T/I16A double mutant of 5-hydroxyisourate hydrolase in complex with T4

Released:
DOI: 10.2210/pdb3q1e/pdb
PDB entry 3q1e coloured by chain, front view.
PDB entry 3q1e coloured by chain, side view.
PDB entry 3q1e coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Probing the evolution of hydroxyisourate hydrolase into transthyretin through active-site redesign.
Cendron L, Ramazzina I, Percudani R, Rasore C, Zanotti G, Berni R
J Mol Biol 409 504-12 (2011)
PMID: 21515285

Function and Biology Details

Reaction catalysed: 
5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-hydroxyisourate hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 119 amino acids
Theoretical weight: 13.17 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06S87 (Residues: 20-138; Coverage: 86%)
  • Best match: Q06S87-2 (Residues: 1-95)
Gene name: urah
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

1 bound ligand:
Ligand T44 2 x T44
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 45.771Å b: 103.462Å c: 53.49Å
α: 90° β: 109.47° γ: 90°
R-values:
R R work R free
0.225 0.222 0.271
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural aspects of thyroid hormone binding to proteins and competitive interactions with natural and synthetic compounds.
Schweizer et al. (2017)
1 more