› 2j6w

X-ray diffraction
2.6Å resolution

R164N mutant of the RUNX1 Runt domain

Released:
DOI: 10.2210/pdb2j6w/pdb
PDB entry 2j6w coloured by chain, front view.
PDB entry 2j6w coloured by chain, side view.
PDB entry 2j6w coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A mutation in the S-switch region of the Runt domain alters the dynamics of an allosteric network responsible for CBFbeta regulation.
Li Z, Lukasik SM, Liu Y, Grembecka J, Bielnicka I, Bushweller JH, Speck NA
J Mol Biol 364 1073-83 (2006)
PMID: 17059830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Runt-related transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03347 (Residues: 46-185; Coverage: 31%)
Gene names: Aml1, Cbfa2, Pebp2ab, Runx1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 91.948Å b: 46.497Å c: 63.552Å
α: 90° β: 90.66° γ: 90°
R-values:
R R work R free
0.214 0.209 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Allosteric regulation and catalysis emerge via a common route.
Goodey et al. (2008)
2 more

1 mention without citation

Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL.
Della Gatta et al. (2012)